Figure 7.
Ibuprofen rescues fAβ-elicited phagocytosis in a proinflammatory milieu. A, BV-2 cells were treated with racemic ibuprofen at indicated doses and IL-1β (15 ng/ml) overnight before stimulation with fAβ25–35 (60μm) for 30 min, followed by a 30 min incubation with fluorescent microspheres. The fraction of phagocytically active cells was then determined. B, BV-2 cells were treated with racemic ibuprofen, S-ibuprofen, or R-ibuprofen at indicated doses together with IL-1β (15 ng/ml) overnight. Cells were then stimulated with fAβ25–35 (60 μm) for 30 min before incubation with fluorescent microspheres for 30 min. The phagocytically active cells were then measured. C, BV-2 cells were treated with IL-1β (15 ng/ml) overnight beforea 1 h incubation with CAY10404, a COX-2 inhibitor, at indicated doses or SC-560, a COX-1 inhibitor, at indicated doses. Cells were then stimulated with fAβ25–35 (60μm) for 30 min before the addition of fluorescent microspheres for 30 min. The fraction of phagocytically active cells was then determined. *p < 0.01, **p < 0.001, and #p < 0.05 compared with control. These data are representative of three independent experiments.