Figure 2.

Histological manifestations of EAE induced by MOG peptide 35–55. A–D, The effect of GA treatment in sagittal brain sections of YFP2.2 mice expressing YFP (green) on their neuronal population. A, Deterioration and transaction of YFP-expressing fibers in the cerebellum and correlation with perivascular infiltration. The insets indicate areas with perivascular infiltrations, demonstrated by staining with antibodies for the T-cell markerCD3.B, Elimination of fibers in lesions in the striatum. Note the thin layer of YFP-positive fiber, frequently found over the lesions in GA-treated mice. C, Typical morphology of pyramidal cells in layer 5 of the cerebral cortex. The arrows and inset indicate abnormal neuronal cell bodies with marginalized nuclei in EAE mice. EAE was induced in YFP2.2 mice, 35 d before perfusion. GA treatment was applied by eight daily injections, starting immediately after EAE induction (prevention). Considerably less damages were found in the brains of EAE+GA mice than in the brains of untreated EAE mice (i.e., less deteriorating fibers, reduced number of lesions with smaller magnitude, and less swollen cell nuclei). D, Staining with Fluoro-Jade B (green), which binds to degenerating neurons, in the cortex of C57BL/6 mice 25 d after disease induction. Figures represent five mice in each group. Scale bar: A, 500 μm; B, C, 50 μm; D, 20 μm. L-2, L-5, and L-6, Layers two, five, and six of the cerebral cortex.