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. 2005 Sep 7;25(36):8217–8228. doi: 10.1523/JNEUROSCI.1859-05.2005

Figure 3.

Figure 3.

Microglial activation in EAE YFP2.2 mice. A, Correlation of the expression of the microglia and macrophage marker MAC-1 (red) with deterioration and injury of YFP-expressing fiber (green) in the white matter of the cerebellum. In box I, highly activated microglia cells are observed, accompanied by reduction in fiber density, whereas in the nearby area in box II, low MAC-1 expression and normal fiber appearance are present. B, The effect of GA on MAC-1 expression and on microglial cell morphology in various brain regions of EAE mice: striatum, thalamus (dorsal lateral geniculate nucleus), and hippocampus (granular and molecular layers). Increased MAC-1 staining and cell morphology typical for activated microglia were displayed in brains of EAE mice (insets). In contrast, MAC-1 expression in brains of EAE+GA mice was reduced extensively, exhibiting cell morphology similar to that of unactivated microglia in naive mice. EAE was induced in YFP2.2 mice 35 d before perfusion. GA treatment was applied by eight daily injections, starting immediately after EAE induction. Representative figures of four to five mice in each group (sagittal sections). Scale bar: A, 500 μm; B, 100 μm in the striatum and thalamus, 50 μm in the hippocampus.