Figure 8.

Fate tracing of neuronal progenitor cells generated in the course of GA treatment in EAE mice. BrdU-incorporated cells (red), born during the concurrent injections of BrdU and GA migrated to various brain regions and expressed neuronal markers. A, B, BrdU-positive cells coexpressing the immature neuronal marker DCX (green) 10 d after the last injection in the striatum (A) and the nucleus accumbens (B). Note the clusters of double-positive cells, suggesting local divisions. C, Staining of DCX-expressing cells in the nucleus accumbens with the endogenous proliferation marker phosphohistone (blue), showing DCX-positive cells that had proliferated in situ before the mouse was killed. D–G, BrdU-positive cells coexpressing the mature neuronal marker NeuN (green) 1 month after completion of GA/BrdU injections in the striatum (D, F), nucleus accumbens (E), and cingulate cortex layer 5 confocal image (G). Arrows indicate representative BrdU/NeuN-coexpressing cells. H–K, BrdU-positive cells 1 month after GA/BrdU injection in YFP mice, coexpressing YFP (green) in the cingulate layer 5 (H, I), occipital layer 6 (J), and motor layer 5 (K) of the cortex. Pyramidal cells with characteristic elongated apical dendrites and axons, indicative of mature functional neurons are shown. Representative figures of five GA-treated mice for each time point. G and K are confocal images (sagittal sections). Scale bar: A, B, H, 200 μm; C, D, 100 μm; E, I–K, 50 μm; F, G, 15 μm.