Figure 11.

A, Representative reverse transcription-PCR for PDGF-A (lanes 2-5) and an internal standard glyceraldehyde-3-phosphate dehydrogenase (GAPDH; lanes 6-9) mRNAs in the spinal cord at 6 h and 1 d after a single intrathecal injection of PBS (lanes 2, 4, 6, and 8) or thrombin (1 pmol/mouse; lanes 3, 5, 7, and 9) in mice. Lane 1 is the indicated marker. B, The intensity of the bands was determined in a semiquantitative manner using NIH Image software. Each value for PDGF-A mRNA in thrombin-injected mice was normalized by the value for the respective GAPDH mRNA. The values in thrombin-injected mice are expressed as a percentage of the increase in PBS-injected mice. Each column represents the mean ± SEM of 10 samples. Error bars represent SEM.