Figure 4.

Classification of spine types in WT and p75^NTRIV KO neurons. A, Representative image of a dendrite from a WT neuron showing the different type of dendritic spines. Scale bar, 2 μm. Spine types were classified in three categories, as shown on the right, based on the spine length (L) as well as the ratio between their maximum head (Max_head) and minimum neck (Min_neck) diameter: stubby (type I), mushroom (type II), and thin (type III). B, The top chart shows the proportions of the three spine types for WT (green) and p75^NTRIV KO (red) neurons. The bottom chart shows these differences plotted for apical, mid-apical, and basal compartment separately. In the basal dendritic compartment of p75^NTRIV KO neurons, the proportion of stubby spines is significantly reduced, but the proportion of mushroom spines is significantly higher than in WT neurons. ^*p < 0.05. Error bars represent SEM.