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. 2005 Sep 28;25(39):8967–8977. doi: 10.1523/JNEUROSCI.1115-05.2005

Figure 8.

Figure 8.

Hypoxia–reoxygenation induces activation of a PrP-luciferase reporter gene containing HSE. N18 cells were transiently transfected using lipofectamine with 6 μg of reporter plasmids [praPrP(-2831)-luc, praPrP(-1026)-luc, praPrP(-514)-luc, and pGL2-control] and pCMVβ-gal, and the luciferase activity of these constructs were analyzed. Luciferase activities of each reporter plasmid were measured in triplicate and expressed as fold-inductions after normalization byβ-gal assay. The results show a 10- to 15-fold increase in luciferase activity of praPrP(-2831)-luc and praPrP(-1026)-luc compared with praPrP(-514)-luc and pGL2-control under conditions of maximal induction. The luciferase activity of praPrP(-2831M)-luc shows a threefold increase compared with praPrP(-514)-luc and the pGL2-control.