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. 2005 Sep 28;25(39):8878–8888. doi: 10.1523/JNEUROSCI.2005-05.2005

Figure 2.

Figure 2.

Asynaptic Drosophila da neurons contain minus-end-out microtubule arrays in dendrites. A, A second instar larva (yw; Gal4 109(2)80, UAS-Nod:GFP) expressing Nod:GFP, a minus-end reporter for microtubules, demonstrates Nod:GFP enrichment at the tips of da dendrites (arrows). B, A second instar larva (yw; Gal4–109(2)80, UAS-Nod::GFP/Gal4–109(2)80 UAS-Tau::GFP) heterozygous for both Tau::GFP, a microtubule-binding protein that labels dendrites, and Nod::GFP demonstrates that Nod::GFP accumulates at the ends of dendrites collinear with the dendrite shaft. C, A second instar larva (yw; Gal4 109(2)80, UAS-Nod::GFP) immunolabeled against Nod::GFP (αGFP; green) and Futsch (αFutsch; red) reveals colabeling (yellow) as puncta at the ends of da neuronal dendrites (arrows). D, A second instar larva (yw; Gal4 109(2)80 UAS-myr::RFP/UAS-Nod::GFP) heterozygous for both myr:RFP and Nod::GFP demonstrates Nod:GFP accumulation at the ends of da dendrites (arrows). Scale bars, 50 μm.