Figure 10.

The mitochondrial pathway is not required for p25 production during excitotoxicity. A, CGNs from Bax +/– and Bax –/– littermates were treated with glutamate (Glut.) for 1 and 3 h, and total cellular protein was analyzed for p35 and p25 levels. B, Protein lysate from glutamate-treated CGN cultures was analyzed for DEVD-AFC cleavage activity. Protein from neurons treated with low levels (5 mm) of K⁺ was included as a positive control for caspase activity. Each error bar represents the mean ± SEM of data from three independent experiments.