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. 2005 Sep 28;25(39):8843–8853. doi: 10.1523/JNEUROSCI.2868-05.2005

Figure 4.

Figure 4.

Effect of LPS-induced inflammation on APP processing in the 3xTg-AD mice. A, Immunoblotting of APP, C99, and C83 in PBS- or LPS-treated 3xTg-AD mice, withβ-actin used as a protein loading control. B, Densitometric analysis of steady-state levels of full-length APP, C99, or C83 between PBS- and LPS-treated 3xTg-AD mice. Data are presented as mean ± SEM (n = 4 each group). C, Total Aβ40 and Aβ42 from detergent-soluble and -insoluble fractions of PBS- and LPS-treated 3xTg-AD mice were measured by ELISA. □, PBS-treated 3xTg-AD mice; ▪, LPS-treated 3xTg-AD mice. Data are presented as mean ± SEM (n = 8 each group). D, Fluorescent intensity of 6E10-immunopositive neurons in the cortex and hippocampus was quantitatively analyzed using Quantity One software. Data are presented as mean intensity ± SEM (n = 5 each group). E, Representative immunofluorescent staining of APP and Aβ fragments by 6E10 in the neocortex and hippocampus of 3xTg-AD mice treated with PBS or LPS. a.u., Arbitrary units; HC, hippocampus; CX, cortex.