Figure 6.

Mutations in modulator binding crevice impair modulation by CX614. Current responses, measured in outside-out patches of transiently transfected HEK 293 cells, of wild-type (WT) GluR2 (flop), and point mutations of residues that interact with CX614 are shown. Top traces show impairment of modulation of deactivation (Deact) by 100 μm CX614 with 3 mm quisqualate; bottom traces show impairment of modulation of desensitization (Des). The P494A mutation was measured in a GluR2 (flip) receptor, because the concomitant mutation in GluR2 (flop) was nonfunctional.