Table 1.
Summary of primers used for PCR analyses
|
mRNA |
Spot number |
Accession number |
Sense primer (5′ to 3′) |
Antisense primer (5′ to 3′) |
|---|---|---|---|---|
| Calreticulin | 1 | NM_022399 | ACGAGCCAAGATTGATGACC | TGGCCTCTACAGCTCATCCT |
| grp78/BiP | 2, 3 | M14050 | AGTGGTGGCCACTAATGGAG | TCAAATTTGGCCCGAGTAAG |
| β-Actin | 4, 5, 9, 10 | NM_031144 | GCGGACTGTTACTGAGCTGCG | TTTATTCGGTCTCACGTCAGT |
| grp75 | 6, 8 | S78556 | CAGAGCCCCAAGTAAAGCTG | TCTGTTGCTCACGTCCTGTC |
| HSP70 | 7 | L16764 | GAGTTCAAGCGCAAACACAA | CTCAGACTTGTCGCCAATGA |
| Vimentin | 11, 12 | NM_031140 | TTTAAGCTTGCCGCAGCCTCTATTCCTCA | TTTCTCGAGTCCCGCATCTCCTCCTCGTA |
| Peripherin | 16, 17 | NM_012633 | GCTGGATCTCTGAGGCTGAC | ATGGGGTCATTCTTGAGCAG |
| HSP60 | 18 | X53585 | ACCTGTGACAACCCCTGAAG | TCTTCCTGTTGTCCCCAAAC |
| γTpm3 | 19 | NM_057208 | GCAAAGCACATTGCAGAAGA | TCTCCGCCTCCTTGAGTTTA |
| γ-Synuclein | 20 | NM_031688 | CAGCCAGGTCTCTTCCTCAC | TGAGCCTCTGTGGTTGACTG |
| Uch-L1 | 21 | NM_017237 | GATTAACCCCGAGATGCTGA | AGGGGACAACTTCTCCGTTT |
| 1-Cys Peroxiredoxin (peroxiredoxin 6) | 22 | NM_053576 | TCATGGGGCATTCTCTTTTC | GGGGCGTATAACGGAGGTAT |
| HSP27 | 23 | NM_031970 | TGTCAGAGATCCGACAGACG | TACTGGGGATGGGTAGCAAG |
| hnRNP H′ | 24, 25 | a | AATGGATTGGGTGTTGAAGC | CCTGTGCCCTATTCTTTCCTT |
| Dismutase (superoxide dismutase 1) | 26 | X05634 | GGATTAACTGAAGGCGAGCA | CAATCACACCACAAGCCAAG |
| ATP synthase subunit d | 27 | NM_019383 | GGAAACGATCTGAAGTCCTG | TGCTTCACAGGTTCTCGATG |
| α Enolase 1 | 28 | NM_012554 | GCCTCCTGCTCAAAGTGAAC | TTCAGAGGGCTCCAGACACT |
| Dimethylarginine dimethylaminohydrolase 2 | 29 | NM212532 | GAGGCAACGACTAGGTCTGC | CCGTAGAGACAGCGAAGTCC |
| SP22 | 30 | NM_057143 | CATCCTAGCCAAAGGAGCAG | CCAAAGCCTACTTCGTGAGC |
| ERp29 | 31 | NM_053961 | CCTGAAGATCATGGGGAAGA | ATGGTCTCAGCAGCCTCAGT |
| Peptidyl isomerase A (cyclophillin A) | 32 | NM_017101 | AGCACTGGGGAGAAAGGATT | AGATGCCAGGACCTGTATGC |
| Cofilin 1 | 33 | NM_017147 | TGTGGCTGTCTCTGATGGAG | TTGTCTGGCAGCATCTTGAC |
| αB crystallin | 34 | X60352 | TTCTTCGGAGAGCACCTGTT | TCCGGTACTTCCTGTGGAAC |
| Peroxiredoxin 1 | 35 | NM_057114 | CTTCTGTCATCTGGCATGGA | AAAGAGGCCCCTGAAAGAGA |
| Glyceraldehyde 3-phosphate dehydrogenase | 37 | NM_017008 | GGAGAAACCTGCCAAGTATG | AGACAACCTGGTCCTCAGTG |
| PGK 1 | 38, 39 | NM_053291 | GCAAAGACTGGCCAAGCTAC | CTTCCAGGAGCTCCAAACTG |
| Aldolase C | 40 | X06984 | TGAGCCAAATTGGGGTAGAG | AGAATGCCCTTCTCCTGGAT |
| HSP90 | S45392 | AAGGCTGAGGCAGACAAAAA | CACCCAACCCTGCTATCTGT | |
| γ-Actin | X52815 | GCAGATGGACTGAGCAGGTGCCAGG | CTTTTATTTCTCTTTACACAAT | |
| 12S mitochondrial rRNA |
|
NC_00165 |
CCCAGAGAACATTAGG |
GAGGAGGGTGACGGGCGG |
With the exception of calreticulin and β-actin, primer pairs were used for standard PCR and for quantitative PCR analyses by SybrGreen assays. See Materials and Methods for primers for quantitative analyses of calreticulin and β-actin transcripts. Nomenclature for mRNAs follows the protein designation from MS analyses (Table 2). Spot number refers to that designated from 2D gels in MS analyses (Table 2). Accession number is the GenBank accession number. Alternate protein names are provided in parentheses.