Figure 3.

Mice cloned from neuronal nuclei-derived ES cells. A, Scheme of the production of cloned fetuses and adults. B, C, Pups derived from Cam-1 ES cells by tetraploid complementation. D, E, Pups derived from the Cam-2 ES cells by nuclear transfer. Note that the embryo generated by tetraploid complementation expressed GFP fluorescence in embryonic tissues but not in the placenta (arrows). In contrast, the embryo generated by nuclear transfer showed GFP fluorescence in both the embryo and placenta (arrows). B, D, Bright field; C, E, UV illumination. Scale bar, 1 cm.