Table 1.
Production of ES cell lines from cerebral cortical neurons by nuclear transfer
|
|
Enucleated oocytes |
Embryos developed to |
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Neuronal marker used |
Injected with cerebral neuronal nuclei |
Survived |
With pseudo pronuclei (%)a
|
Two cell (%)b
|
Blastocysts (%)b
|
ES cell lines (%) establishedb
|
|||
| αCaMKII | 587 | 275 | 162 (28) | 117 (72) | 25 (15) | 2 (1) | |||
| Nex | 769 | 419 | 263 (34) | 153 (58) | 31 (12) | 2 (1) | |||
| GAD67 | 670 | 373 | 210 (31) | 133 (63) | 20 (10) | 2 (1) | |||
| Total |
2026 |
1067 |
635 (31) |
403 (63) |
76 (12) |
6 (1) |
|||
a
The percentage of injected oocytes, determined 5 h after Sr2+ activation.
b
The percentage of oocytes with pseudo pronuclei.