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. 2005 Oct 12;25(41):9367–9377. doi: 10.1523/JNEUROSCI.0849-05.2005

Figure 4.

Figure 4.

NMDA receptor stimulation leads to an inhibition of sAPPα release and an increased Aβ production. a, Immunoprecipitations of sAPPα or sAPPβ and total sAPPs in conditioned media of cultured neurons treated with NMDA (7.5 μm) for 24 h. sAPPα was revealed with the R1736 antiserum, sAPPβ with the 192 antiserum, and sAPPs with the 22C11 antibody after capturing all APP isoforms with the 22C11 antibody. sAPPx/total sAPP ratios were estimated by densitometry. Open bars, sAPPα; filled bars, sAPPβ. Results are the mean of four experiments performed in triplicate. Statistical analysis was realized by ANOVA followed by Bonferroni-Dunn's test (n = 12; *p < 0.001 to control; #p < 0.02 to NMDA). b, Western blot analysis of extracellular Aβ in neurons after NMDA application for 24 h by using antisera raised against either the C-terminal extremity of Aβ42 (R1742) (top) or Aβ1-10 (R600) (bottom). p3 levels and Aβ levels were estimated by densitometry. Open bars, p3; filled bars, Aβ. Results are the mean of four experiments performed in triplicate. Statistical analysis was realized by ANOVA followed by Bonferroni-Dunn's test (n = 12; *p < 0.001 to control; #p < 0.001 to NMDA). c, Quantitative determination of Aβ production by ELISA from the same extracts used in b. d, Western blot analysis of intracellular Aβ in neurons after NMDA application for 24 h by using R600 antiserum. Actin levels are shown in the bottom blot. IP22C11, Immunoprecipitated with 22C11; MW, molecular weight.