Figure 6.

KPI-dependent Aβ₄₂ production in cortical neurons is reversed by the coincubation of a blocking antibody raised against the KPI domain. a, Immunoprecipitation of sAPPα generation in conditioned media of cultured neurons treated with NMDA (7.5 μm) in the presence of an antibody raised against either the KPI domain of APP or the N terminus of APP (residues 66-81) for 6 h. sAPPα levels were estimated by densitometry. Results are the mean of three experiments performed in triplicate. Statistical analysis was realized by ANOVA followed by Bonferroni-Dunn's test (n = 9; ^*p < 0.01 to control). b, Western blot analysis of Aβ₄₂ generation in neurons in the same protein samples used in a. Note that Aβ was immunoprecipitated and revealed with the FCA3542 antiserum against Aβ₄₂. Levels of Aβ were estimated by densitometry. Statistical analysis was realized by ANOVA followed by Bonferroni-Dunn's test (n = 9; ^*p < 0.01 to control). IP 22C11, Immunoprecipitated with 22C11; WB, Western blot.