Figure 7.

TBI enhances Aβ production. a, Using membrane preparations, total APP and APP-CTFs levels were determined by Western blot using APPCter-C17 in three to four animals per group. KPI-APP expression was slightly increased 6 h after TBI and was clearer at 24 h after trauma. In parallel, amounts of CTF-α were reduced, whereas amounts of CTF-β were increased above the threshold of the detection limit. β-Tubulin was used as loading control (bottom box). Densitometric analyses of presented immunoblots are displayed to the right (ANOVA; n = 3; ^*p < 0.01 to control). b, Estimation of the amount of sAPPα (top blot) and Aβ (bottom blot) after TBI. Densitometric analyses of presented immunoblots are displayed to the right (ANOVA; n = 3; ^*p < 0.05 to control or to the ipsilateral signal at 6 h after trauma for Aβ). C, Control; I, tissue samples ipsilateral to the impact; 6, 6 h after trauma; 24, 24 h after trauma. WB, Western blot.