Table 1.
Molecular testing of in vivo recorded CCK-expressing interneurons by immunocytochemistry
|
|
CCK-immunopositive interneurons |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Molecules tested |
T64c (basket) |
T147a (basket) |
T152b (basket) |
T165d (basket) |
T46d (apical dendrite Innervating) |
T159a (apical dendrite innervating) |
T82e (perforant path associated) |
T123b (perforant path associated) |
T99d (unclassified) |
||||||||
| CCK | + | + | + | + | + | + | + | + | + | ||||||||
| VIP | − | − | + | + | − | − | + | ||||||||||
| SOM | − | ||||||||||||||||
| NPY | − | − | − | ||||||||||||||
| CRF | − | ||||||||||||||||
| PPTB | + | + | − | ||||||||||||||
| VGLUT3 | + | − | + | − | − | ||||||||||||
| GAD | + | + | + | + | + | ||||||||||||
| Parvalbumin | − | − | − | − | − | − | − | ||||||||||
| Calbindin | − | − | − | + | − | ||||||||||||
| Calretinin | − | − | − | − | − | ||||||||||||
| CB1 rec | + | + | + | + | |||||||||||||
| mGluR1α | − | − | weak+ | − | − | − | − | − | |||||||||
| Input mGluR7a | − | ||||||||||||||||
| Input mGluR8a | − | ||||||||||||||||
| GABAAα1 | − | − | − | − | − | ||||||||||||
| NK1 rec | + | + | − | − | − | ||||||||||||
| NOS |
|
− |
− |
− |
|
− |
|
− |
|
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Cells were tested by immunofluorescence for up to 15 molecules on soma, dendrites, and axonal boutons in separate sections or by rereacting the same section if the cell had been immunonegative. The input terminals of one cell were tested for mGluR7a and mGluR8a. +, Immunopositive;−, immunofluorescence undetectable in cell, but nearby cells show immunoreactivity; vacant positions, cell not tested. rec, Receptor;NK1, neurokinin-1; NOS, nitric oxide synthase.