Figure 1.

Increased expression of Fyn triggers hAPP/Aβ-induced depletion of calbindin in the dentate gyrus. A, Immunostaining for calbindin in the molecular layer of the dentate gyrus (arrow) demonstrates marked depletion in a severely affected FYN/hAPP_low mouse compared with NTG and singly TG mice. B, C, Quantitation of relative calbindin immunoreactivity (IR) levels in the molecular layer of the dentate gyrus in 23-37 mice per genotype (B) and 9 mice per genotype (C). The interaction between hAPP_low and FYN was significant by ANOVA (p < 0.001). ^**p < 0.01, ^***p < 0.001 versus NTG; ^#p < 0.05. D, Western blot analysis of dentate gyrus lysates demonstrates comparable levels of hAPP (top) and Fyn (middle) overexpression in singly TG and FYN/hAPP_low mice, as well as calbindin reductions in FYN/hAPP_low mice (bottom). Scale bar, 250 μm.