Figure 2.

hAPP/Aβ and Fyn cooperate to diminish the number of Fos-positive granule cells in FYN/hAPP_low mice. A, Immunostaining for Fos demonstrates a typical pattern of granule cell labeling (arrow) in the dentate gyrus of an NTG mouse and different degrees of Fos reduction in TG mice. FYN/hAPP_low mice were affected most severely. B, Calbindin levels correlated with the number of Fos-positive granule cells in singly and doubly TG mice but not in NTG controls. The p and R² values refer to TG groups only. C, D, Quantitation of Fos-positive granule cells revealed significant reductions in hAPP_low and FYN mice (C). FYN/hAPP_low mice had more severe depletions (C) that were of similar magnitude as those in hAPP_high mice (D). n = 23-37 (C) or n = 9 (D) mice per genotype. ^**p < 0.01, ^***p < 0.001 versus NTG. Scale bar, 100 μm.