Figure 2.

Circadian regulation of VDCC expression in vivo and in vitro. Temporal patterns of P/Q- and T-type calcium channel expression in the rat SCN and cerebellum (A; n = 5) and in SCN2.2 and GH3 cells (B; n = 3). Symbols denote the ratios of P/Q-type (α1A) or T-type (α1G) subunit/CypA mRNA signal. For GH3 cells, P/Q-type calcium channel expression was plotted relative to the secondary ordinant (B). Significant differences between peak mRNA levels for P/Q- and T-type calcium channels and those observed during preceding or succeeding minima (p < 0.05) are denoted separately for the SCN (^*) and cerebellum (†).