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. 2005 Nov 2;25(44):10252–10261. doi: 10.1523/JNEUROSCI.3348-05.2005

Figure 2.

Figure 2.

Effect of Colivelin on neuronal cell death induced by V642I-APP. A, F11 cells were transfected with the V642I-APP expression vector and then treated with various concentrations of Colivelin or AGA-(C8R)HNG17 (100 am, 1 fm, 10 fm, 100 fm, 1 pm, 10 pm, 100 pm, 1 nm, 10 nm, or 100 nm). Seventy-two hours after transfection, cell mortality was measured by Trypan blue exclusion assay. As controls, mortality of cells not transfected or transfected with the pcDNA vector was measured. B, Protein expression of V642I-APP was determined by immunoblot analysis with antibody to PS1. C, Roles of CaMKIV and STAT3 in Colivelin-mediated neuroprotection against V642I-APP-induced neurotoxicity. F11 cells, transfected with or without the V642I-APP expression vector together with dnCaMKIV or dnSTAT3 expression vectors, were incubated with or without 100 pm ADNF, AGA-(C8R)HNG17, or Colivelin. Seventy-two hours after transfection, cell mortality was measured by Trypan blue exclusion assay. All experiments performed with n = 3. noT, Not transfected; Vec, vector. Error bars represent SD.