Figure 3.

Effect of Colivelin on neuronal cell death induced by M146L-PS1. A, B, Effect of Colivelin on neuronal cell death induced by M146L-PS1. F11 cells were transfected with the M146L-PS1 expression vector and then treated with various concentrations of Colivelin or AGA-(C8R)HNG17 (100 am, 1 fm, 10 fm, 100 fm, 1 pm, 10 pm, 100 pm, 1 nm, 10 nm, or 100 nm). As controls, mortality of cells not transfected or transfected with the pcDNA vector was measured. Seventy-two hours after transfection, cell mortality was measured by Trypan blue exclusion assay (A). Protein expression of M146L-PS1 was determined by immunoblot analysis with antibody to PS1 (B). All experiments were performed with n = 3. noT, Not transfected; Vec, vector; holo, holoprotein; NTF, N-terminal fragment. Error bars represent SD.