Effect of Colivelin on death induced by treatment with 20 μm glutamate in PCNs. PCNs were treated with 20 μm glutamate in association with various concentrations of AGA-(C8R)HNG17, ADNF, or Colivelin (1 fm, 100 fm, 10 pm, or 1 nm). Seventy-two hours after the start of treatment, cells were measured with WST-8 assay (n = 3). Error bars represent SD. Statistical analysis was performed by one-way ANOVA followed by Fisher's PLSD (*p<0.05, **p<0.01).