Immunohistochemical analysis of ChAT-immunoreactive neurons in the medial septum. A-C, Coronal sections of medial septa of mice repetitively infused with Aβ25-35 together with or without Colivelin treatment were immunohistochemically stained with anti-ChAT antibody. Scale bar, 100 μm. D, The average numbers of ChAT-immunoreactive [ChAT (+)] neurons in the medial septa of five coronal sections, 10 μm in thickness (total, 50 μm thickness), were compared (n = 3). Data are shown as means ± SEM. Statistical analyses were performed by one-way ANOVA followed by Fisher's PLSD (**p < 0.01).