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α-LTxN4C is almost inactive in stimulating NE release. A, Analysis of purified recombinant α-latrotoxins. The recombinant α-latrotoxins (GST-cleaved α-LTxN4C and α-LTxWT; ∼1-2 μg) were analyzed by SDS-PAGE and Coomassie staining. B, Stimulation of NE secretion from PC12 cells with 10 nm α-LTxN4C and α-LTxWT. Error bars indicate SEM (n = 6-12).
