Effects of the deletion of ankyrin-like repeats from recombinant α-latrotoxin on binding to neurexin 1α and CL1. A, Schematic representation of recombinant α-latrotoxins, the binding of which to neurexin 1α and CL1 was examined. C's indicate the three cysteine residues that are conserved in all latrotoxins. B, C, Immunoblot analysis of the bound recombinant α-latrotoxins on immobilized control proteins (IG-C), neurexin 1α (IG-N1α-31), and CL1 (IG-CL1-1). Bound α-latrotoxins were probed by monoclonal antibody against c-myc.