Figure 2.

Allele-specific silencing of torsinA(ΔE) in a simulated heterozygous state. Cos7 cells were transiently transfected with torsinA(wt)-GFP, HA-torsinA(ΔE), and different pVETL-(GFP)shRNAs. A, Schematic diagram of pVETL constructs used for expression of shRNAs and the GFP reporter. B, Western blot analysis showing allele-specific suppression of HA-torsinA(ΔE) by shTAmut5, and potent suppression of both forms of torsinA by shTAcom. C, Quantification of three experiments as in B. Error bars indicate SE. mis, U6shTAmis; com, U6shTAcom; mut5, U6shTAmut5; torA, torsinA; tub, α-tubulin; WB, Western blot.