Figure 2.

Three-dimensional rotation and quantitation of intact and disassembled endplates of muscle fibers expressing control plasmid or kinase-inactive Src. A-J, 3D reconstructions as shown in Figure 1 B were rotated around the x-axis to illustrate the relative positioning of nerve, AChR clusters, and GFP-stained nuclei. K, L, In experiments as described in Figure 1, the degree of NMJ disassembly was scored as detailed in Materials and Methods. Control fibers score mostly as intact endplates with some partial and no complete disassembly (dis.). Fibers expressing Src-AM (K) or Src-K295M (L) show a high percentage of complete and partial disassembly compared with control. Disassembly is quantified as a percentage of all endplates analyzed within the control, Src-AM, and Src-K295M groups. Fifty endplates were analyzed for each group.