Figure 5.

Synaptic vesicle proteins disperse with different kinetics after stimulation. A, GFP-α-synuclein (GFP αsyn) and GFP-A53T-α-synuclein (data not shown) disperse more slowly than GFP-synapsin (GFP-syp) after 600 action potentials (AP) delivered at 10 Hz. In contrast, GFP and GFP-A30P-α-synuclein (GFPA30P) do not disperse after 600 AP, consistent with their behavior as soluble proteins. The kinetics of α-synuclein dispersion most closely resembles that of GFP-SV2. The traces shown are averages ± SEM of 22-54 boutons from one representative cell for each construct. B, Average maximal dispersion of GFP-tagged proteins immediately after 600 AP. Error bars indicate SEM (n > 300 boutons from 6-9 cells for each construct).