Figure 6.

Acute energy inhibition increases cerebral Aβ40 levels in Tg2576 mice. Brain homogenates from Tg2576 mice treated with 18 U/kg insulin, 1 g/kg 2DG, 100 mg/kg 3NP, 30 mg/kg KA, or vehicle were subjected to Aβ40-specific sandwich ELISA. Recovery times were for 4 h (A), 2 d (B), or 7 d (C). Aβ40 levels were expressed as picograms per milligram of total brain protein (determined by BCA assay). Note that brain Aβ40 levels showed a trend toward elevation for several of the treatments after 4 h and 2 d of recovery but did not reach significance. However, by 7 d of recovery, brain Aβ40 levels were increased approximately twofold by all treatments. Data represent mean ± SEM (^**p < 0.01 and ^***p < 0.001, one-way ANOVA with Newman-Keuls multiple-comparison test; n = 4 per treatment).