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Inhibition of PI3K and Akt reduces dendritic tree complexity. A, Representative micrographs of hippocampal neurons transfected with Bcl-2 at 7 DIV and treated for 3 d from 9 DIV with either 50 μm LY294002 or 10 μm Akt inhibitor or 25 μm Akt inhibitor III. Neuron morphology was visualized by cotransfected GFP. B, Sholl analysis of hippocampal neurons treated with PI3K or Akt inhibitors or DMSO vehicle as in A.
