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Rapamycin inhibition of PI3K effect can be circumvented by rapamycin-insensitive mutant of mTOR. A, Representative micrographs of hippocampal neurons transfected at 7 DIV with the indicated constructs and treated with vehicle or rapamycin (10 nm) for 6 d. Neuronal morphology was visualized by cotransfected GFP. B–E, Sholl analysis of neurons transfected with the indicated constructs in the absence or presence of rapamycin (+Rapa).
