Skip to main content
. 2005 Dec 7;25(49):11349–11356. doi: 10.1523/JNEUROSCI.3328-05.2005

Figure 1.

Figure 1.

Kisspeptin exerts a potent, direct depolarizing action on GnRH neurons that is regulated developmentally. Perforated-patch, voltage recordings from GnRH–GFP neurons in the acute brain slice preparation of male mice. A, An adult male GnRH neuron (RMP, –63 mV) is depolarized by 100 nm kisspeptin-10 (KP-10) for >20 min and exhibits short bursts of action potentials (inset). B, Adult male GnRH neuron (RMP, –60 mV) exhibiting a depolarizing response and pronounced membrane oscillations to 10 nm kisspeptin-10 (KP-10) in the presence of TTX. This cell continued to display membrane oscillations for >50 min before the recording was lost. C, A prepubertal (P30; RMP, –63 mV) GnRH neuron exhibiting a depolarizing response associated with increased firing in response to 100 nm kisspeptin-10 (KP-10). The depolarizing response in this cell lasted for 20 min. D, Spontaneously active juvenile (P9; RMP, –60 mV) GnRH neuron responding to kisspeptin-10 (KP-10) with a transient depolarization. E, Developmental recruitment of kisspeptin-sensitive GnRH neurons across puberty. The histogram shows the percentage of male juvenile (P8–P19), prepubertal (P26–P33), and adult (older than P60) GnRH neurons activated by KP-10 (n in parentheses). PND, Postnatal day.