Figure 2.

Kisspeptin exerts a potent activational effect on GnRH neurons in adult female proestrous mice. Perforated-patch, voltage recordings from proestrous female GnRH–GFP neurons in the acute brain slice demonstrate a remarkably intense and prolonged activation by 10 nm kisspeptin (KP-10). A, Adult female GnRH neuron (RMP, –75 mV) that was activated by KP-10 for >80 min, at which time the recording was lost. B, Adult female GnRH neuron (RMP, –68 mV) activated by KP-10. The firing pattern occurring within the open box is shown at an expanded time scale on the right.