Figure 6.

Late inflammatory response to LPS in CNS of chimeric mice. Representative film autoradiographs (a-p) show proinflammatory mRNA induction in brains of mice killed 6 h after intraperitoneal LPS. IκBα, IL-1β, TLR2 (i-l), and CD14 (m-p) mRNAs were detected by radioactive in situ hybridization on brainstem sections from chimeric mice: HeJ → HeJ (a, e, i, m), WT → WT (b, f, j, n), WT → HeJ (c, g, k, o), and HeJ → WT (d, h, l, p). Quantified expression levels are shown (q-t). IκBα (q), IL-1β (r), TLR2 (s), and CD14 (t) mRNA levels are plotted as mean integrated density ± SEM from three or four mice per chimeric class. Individual group means were compared against the control WT → WT group, and statistical significance was established by one-way ANOVA and appropriate post hoc tests. ^*p < 0.05; ^**p < 0.001.