Figure 5.

Kinetics of exploration-induced Arc protein expression in the DG. A, Example of a reconstructed 20 μm flat image, as was used for analysis of Arc expression in granule cells of the DG. Note the sparsity of Arc staining (red) across the entire DG, with the sparsest expression observed in the lower blade. B, Percentage of cells stained for Arc protein in the upper and lower blades of the dentate gyrus at all time points studied. C, Percentage of cells stained for Arc cytoplasmic mRNA in the upper and lower blades of the dentate gyrus at all time points studied. ^*p < 0.0014 compared with controls. Scale bar, 100 μm.