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Knockdown of SDF-1a and CXCR4b led to pathfinding errors by RGC axons. All panels are ventral views of retinas of 48 hpf ath5:gfp transgenic embryos. A, The optic nerve and RGCs marked by GFP fluorescence in a 48 hpf ath5:gfp transgenic embryo injected with 2 ng of control MO. B, C, Injection of 1 ng each of sdf-1a and cxcr4b antisense MOs leads to pathfinding errors by RGC axons (arrows). Scale bar, 20 μm.
