Skip to main content
. 2005 Feb 16;25(7):1718–1729. doi: 10.1523/JNEUROSCI.4835-04.2005

Figure 5.

Figure 5.

BIIE0246 (BIIE; 100 nm) essentially blocked the effect of NPY (1 μm) and enhanced recurrent mossy fiber synaptic transmission when applied by itself. Its effects were restricted to recurrent mossy fiber synapses. A, Recordings from the experiment shown in B are NMDA receptor-mediated compound recurrent mossy fiber EPSCs and are the averages of 10 traces. BIIE0246 increased response amplitude when applied by itself. The addition of NPY to the superfusion medium in the continued presence of BIIE0246 hardly affected the size of the response. See Figure 3A for other details. B, During this representative experiment, a hippocampal slice was superfused sequentially with BIIE0246 (•), BIIE0246 plus NPY (▴), and finally BIIE0246 plus NPY plus 50 μm d-AP-5 (▪). A 0.1 Hz train of 10 stimuli was applied to the mossy fibers just before the addition of BIIE0246 to the superfusion medium (○) and after complete equilibration of the slice with each modified superfusion medium. For each response, peak amplitude is plotted against the time during the experiment at which it was recorded. Note that in the presence of BIIE0246 alone, the amplitude of the response to the first stimulus in the train was approximately the same as the amplitude of the succeeding responses. Thus, BIIE0246 enhanced response amplitude in the absence of a prepulse. BIIE0246 also attenuated the effect of NPY. This experiment was replicated three times with similar results. C, Grouped data (means ± SEM) obtained from seven cells exposed to NPY alone, six cells exposed to BIIE0246 alone, and five cells exposed simultaneously to NPY and BIIE0246. *p < 0.01 by paired t test with respect to responses recorded just before the application of the test compound(s). D, BIIE0246 increased the peak amplitude of the NMDA receptor-mediated compound recurrent mossy fiber (MF) EPSC but not that of similar responses evoked by stimulation of perforant path (PP) or associational-commissural (AC) fibers. Values are means ± SEM for six (mossy fiber), four (perforant path), or three (associational-commissural) granule cells. *p < 0.01 by paired t test with respect to responses recorded just before exposure to BIIE0246.