Figure 4.

CaMKK and ERK mediate E-LTP via a common mechanism. A, B, Mouse hippocampal slices were treated without or with the MEK inhibitor U0126 (UO; 10 μm) and/or the CaMKK inhibitor STO-609 (STO; 5 μm) by bath application as in Figure 2 D for 30 min before and 5 min after E-LTP induction. Control (CTL), n = 24; U0126, n = 10; STO-609, n = 11; U0126 plus STO-609, n = 10. C, D, At various times after E-LTP induction (as in Fig. 2 D), the activation status of ERK (C) and CaMKI, AKT, CaMKII, and Ras-GRF1 (D) was determined at the indicated times. Mean ± SE; n = 5. ^**p ≤ 0.01.