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Codistribution of tSNAREs with α7-nAChR clusters on CG neurons. Freshly dissociated E14 CG neurons were costained with AlexaαBgt for α7-nAChRs (A, D, G, J) and antibodies for SNAP-25 (B), syntaxin 1 (E), SV2 (H), or nonimmune serum as a negative control (K), and the corresponding pairs of fluorescence images were merged (C, F, I, L). Both SNAP-25 and syntaxin 1 codistribute with α7-nAChRs. Similar results were obtained in three additional experiments. Scale bar, 10 μm.
