Figure 7.

Akt phosphorylation and status of caspase-3 in Irs2^-/- retina. The retinas were dissected from 9-week-old WT and Irs2^-/- mice. The whole retinal lysates (50 μg) were separated by 7.5% SDS-PAGE gels. Western blot was used to determine Irs1, IGF1r, p85, and caspase-3 (casp3) (b, c). For analysis of phosphorylation of Akt, the dissected retinas were treated with or without human IGF1 in vitro (100 nm for 20 min), and Western blot was used to detect Akt phosphorylation using antibody against phospho-Akt (pAkt; Ser⁴⁷³) (a). Four retinas were pooled from two mice of each genotyping for each experiment, and two independent experiments were performed.