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. 2005 Feb 9;25(6):1354–1365. doi: 10.1523/JNEUROSCI.3034-04.2005

Figure 2.

Figure 2.

Myelin deficits in Olig1-null mice. A, Expression of myelin genes analyzed by in situ hybridization using probes to Olig1, Mbp, and Plp1/Dm-20 and immunocytochemistry using anti-CNP antibodies on frozen sections of P14 brains from Olig1-heterozygous (+/-) or -null (-/-) mice. Expression of Mbp, Plp1/Dm-20, and CNP is evident in white matter tracts (arrows) of +/- but not -/- mice. B, In situ hybridization signals for mature oligodendrocyte markers, NogoA and Omgp, are absent in -/- mice (black arrows); however, expression in neurons is unaffected (red arrowheads). C, Myelin lipid deposition around axons is detected using Gallyas silver staining in white matter tracts of +/- mice (left column; arrows) but not -/- mice (right column). Coronal and parasagittal sections of forebrain and cerebellum are shown. Ctx, Cortex; cc, corpus callosum; St, striatum.