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. 2005 Feb 9;25(6):1442–1447. doi: 10.1523/JNEUROSCI.4041-04.2005

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Copyright © 2005 Society for Neuroscience 0270-6474/05/251442-06.00/0

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Figure 4. — Expression of BH3-only proteins after axotomy. A, Expression of BH3-only protein family members 7 d after axotomy in rats and mice was examined by in situ hybridization (right is operated side; arrows). Scale bars: top, 1.2 mm; bottom, 0.7 mm. B, RT-PCR analysis of BH3-only protein members in control (c) and operated (o) hypoglossal nuclei of mice 7 d after nerve injury. Noxa appeared to comprise a large proportion of the BH3-only protein family members in injured motor neurons of mice. C, Quantification of results of the RT-PCR shown in B. The density of each band was normalized against that of GAPDH. Data represent mean values ± SD from three independent experiments (p < 0.001 compared with the results of other BH3-only proteins; ANOVA).