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. 2005 Dec 14;25(50):11495–11503. doi: 10.1523/JNEUROSCI.3697-05.2005

Figure 4.

Figure 4.

FcRn-dependent 125I-Aβ40–4G8 BBB clearance in old APPsw+/– mice and FcRn BBB expression in AD. A, 125I-Aβ40 (40 nm) BBB and ISF clearance in 18- to 20-month-old APPsw+/– (Tg2576) mice 30 min after brain ISF microinjections and 120 h after vehicle or 4G8 intraperitoneal treatment, as in Figure 2 A. B, The BBB clearance of 125I-Aβ40 or 125I-Aβ40–4G8 complexes (40 nm) in 20- to 23-month-old Tg2576 mice within 30 min of brain ISF microinjections. C, 125I-Aβ40–4G8 (1 nm) uptake by isolated cerebral microvessels of Tg2576 mice at 3–5 and 20–23 months of age determined within 1 min at 37°C without and with Fc fragment (10 μm) and RAP (5 μm). D, FcRn colocalization with vWF (endothelial intracellular marker) in the brain of 10-month-old Tg2576 mouse studied by confocal microscopy. E, Western blot analysis of FcRn in brain microvessels of control and Tg2576 mice at 5–7 and 20–23 months of age (top) and its relative abundance by scanning densitometry corrected for β-actin (bottom). F, FcRn and vWF double immunostaining in brains of AD and age-matched individuals (left) and FcRn-positive vascular profiles (right). Scale bar, 50μm. Mean ± SEM; n = 3–6.