Figure 5.

Visual deprivation in juvenile mice alters the NR2A/B ratio in the PSD, but only in PNS and LSM fractions in adult mice. A, Biochemical fractionation progressively enriches NR2A, NR2B, and PSD-95 and eliminates a nonsynaptic protein, β-tubulin, invisual corticals amples from deprived and control adult mice. Samples of 10μg were loaded into each gel lane. B, Quantification of NR2A and NR2B band intensities, which were normalized to the value at 12.5μg. The inset is a representative NR2A/B immunoblot of a PSD fraction. Samples of 1–12μg were loaded into each gel lane; results from three blots were averaged. C, NR2A/B ratios were measured in PNS, LSM, and PSD fractions of visual cortices of deprived and control from both juvenile and adult mice. The values (means±SEM) are normalized to average control values. ^*p < 0.05.