Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Feb 23;25(8):2002–2009. doi: 10.1523/JNEUROSCI.4474-04.2005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005 Society for Neuroscience 0270-6474/05/252002-08.00/0

PMC Copyright notice

Figure 5. — Parkin mediates K63-linked polyubiquitination of synphilin-1. A, A portion of lysates prepared from HEK 293 cells transfected with FLAG-tagged parkin alone or with various HA-tagged ubiquitin species were subjected to anti-FLAG immunoprecipitation followed by anti-HA immunoblotting (left panel). The blot was stripped and reprobed with anti-FLAG to show the amounts of immunoprecipitated parkin (top right panel). Lysates prepared from these variously transfected cells (INPUT) were also subjected to anti-FLAG immunoblotting to show FLAG-parkin expression levels (bottom right panel). B, A portion of lysates prepared from HEK 293 cells transfected with the indicated combinations of myc-tagged synphilin-1, FLAG-tagged parkin, and various HA-tagged ubiquitin species were subjected to anti-myc immunoprecipitation followed by anti-HA immunoblotting (top left panel). The blot was stripped and reprobed with anti-myc (bottom left panel) to illustrate that relatively equal amounts of synphilin were immunoprecipitated. The remaining portion of the transfected cell lysates was immunoblotted with anti-HA (top right panel) and anti-myc (bottom right panel) to show their respective expression levels. These experiments were replicated at least three times.