Figure 2.
Western blotting for Trx and Hsp72 in murine retinal samples. A, Expression of Trx and Hsp72 in GGA-untreated [GGA(-)] and GGA-treated [GGA(+)] mice in RPE fraction. B, Expression of Trx and Hsp72 in GGA-untreated [GGA(-)] and GGA-treated [GGA(+)] mice in the neural retina. Samples from two independent mice in each group were analyzed by Western blotting in each experiment. The experiments were repeated three times, and the band intensities of GGA+ samples relative to GGA-samples are shown in the bar graph (mean ± SD; n = 6). Sample loading was monitored by staining with Coomassie Brilliant Blue R-250 (CBB).