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. Author manuscript; available in PMC: 2020 Jun 21.
Published in final edited form as: ACS Chem Biol. 2019 May 13;14(6):1097–1101. doi: 10.1021/acschembio.9b00069

Figure 2.

Figure 2.

Characterization of ClbPFL activity in vitro. (A) LC-MS detection of the prodrug scaffold ([M + H]+: 343.2597 m/z) produced by ClbP-mediated hydrolysis of 100 μM 2 in vitro. Error bars represent 1 standard deviation (SD), n = 3. (B) SAR study of ClbP substrates. a Based on detection of N-acyl-d-Asn cleavage products by LC-MS. Figures S4-S7.