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Fluorogenic probes monitor ClbP activity in whole cells and in vitro. (A) Mechanism of activation of fluorogenic probes. (B) 17 is activated by ClbPFL but not ClbPpep or ClbPFL-S95A in vitro. Points represent mean fluorescence of 6 reactions for each enzyme ± SD. (C) 17 can be activated by whole cells. Points represent mean fluorescence of 6 cultures of E. coli BL21 expressing each enzyme ± SD.
